Galvani, L.  De viribus electricitatis in motu musculari commentarius (1792).

In the course of his experiments on irritable responses caused by static electricity applied to frog muscles, Galvani (1737-1798) produced electric current from the contact of two different metals in a moist environment, producing what became known as the galvanic response.  He first noticed the reaction when he fastened some prepared frogs by "brass hooks in their spinal cord to an iron railing which surrounded a certain hanging garden on my house."  Galvani mistakenly believed this phenomenon (which his nephew called "galvanism") to be evidence of animal electricity, an 18th conception that animal nerves and muscles contained a subtle fluid similar to electricity.  Galvani used prepared frogs consisting of the spinal cords, crural nerves and lower limbs.  The first publication of Galvani's results was in 1791 in the journal Bononiensi scientiarum et artium instituto atque academia commentarii of Bologna (vol.7, pp.363-418); this publication is what appears in Garrison & Morton.

(Sources:  Garrison & Morton; Dictionary of Scientific Biography)